Skip to main content
Fig. 7 | Genome Biology

Fig. 7

From: Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems

Fig. 7

Workflow for the visual screening of stably inherited gene-edited mutants in Arabidopsis with the assistance of p19. The pIUC-p19 expression vector is used for Arabidopsis transformation via the Agrobacterium-mediated flower-dipping method. T1 plants are grouped into three types according to the severity of p19-induced leaf phenotypes. Type III plants will have a high probability of containing CRISPR/Cas9-induced mutations therefore analysis should be focused on these plants. T2 plants derived from gene-edited T1 seedlings are grouped again based on the p19-induced leaf phenotypes. Segregation of the inserted T-DNAs is easily recognized in this generation looking for wild-type looking plants. Screening for homozygous mutants without T-DNA inserts should be performed focusing on wild-type plants

Back to article page