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Fig. 6 | Genome Biology

Fig. 6

From: Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems

Fig. 6

Establishing the role of p19 in CRISPR/Cas9 efficiency in Arabidopsis. a Schematic diagram of the p19 over-expression vector pOE-p19 used to transform CT-#4 lines of different mutant backgrounds. BASTA: bar resistance gene. b Relative p19 expression in pOE-p19 T2 transgenic plants of different CT-#4 backgrounds detected by quantitative RT-PCR. The columns indicate mean ± SD of three biological replicates. c sgRNA, siR-Cas9, and miR167 transcript levels in pOE-p19 T2 transgenic plants of different CT-#4 backgrounds detected by small RNA Northern blot. The U6 expression levels were used as internal reference. d Relative Cas9 expression levels in pOE-p19 T2 transgenic plants of different CT-#4 backgrounds detected by quantitative RT-PCR. The columns indicate mean ± SD of three biological replicates. eh Relative integrity of the GUUS reporter gene in F1 hybrids of p19 overexpressing CT-#4 and GR-#16 lines in Col-0 (e), ago1-27 (f), ago2-1 (g) and ago4-6 (h) backgrounds detect by real-time PCR. The columns indicate mean ± SD of three technical replicates. **Significant difference at p < 0.01 under two-tailed Student’s t test

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