Fig. 3
From: A novel long noncoding RNA HOXC-AS3 mediates tumorigenesis of gastric cancer by binding to YBX1
HOXC-AS3 regulates GC cell proliferation and migration in vitro. a Analysis of HOXC-AS3 expression levels in GC cell lines (SGC-7901, BGC-823, MGC-803, and SNU-601) compared with a normal gastric epithelium cell line (GES-1) by qRT-PCR. b MTT assays were performed to determine cell proliferation of BGC-823 and SGC-7901 cells after transfection of overexpression plasmid and ASO of HOXC-AS3. c EdU assays of BGC-823 and SGC-7901 cells transfected with an overexpression plasmid and knockdown of HOXC-AS3. d Colony formation assays of BGC-823 and SGC-7901 cells transfected with an overexpression plasmid and knockdown of HOXC-AS3. e Transwell assays were used to investigate the changes in migratory abilities of BGC-823 cells after transfection, respectively. f At 48 h after transfection, cell cycle was analyzed by flow cytometry. The bar chart represents the percentage of cells in G1–G0, S, or G2–M phase, as indicated. g At 48 h after transfection, BGC-823 and SGC-7901 cells were stained and analyzed by flow cytometry. LR, early apoptotic cells. UR, terminal apoptotic cells. *P < 0.05, **P < 0.01