Fig. 3

A subset of HMs and AS are strongly associated upon hESC differentiation. a Representative profiles of HM (H3K36me3) changes (normalized Δ reads number) around the inclusion-gain (red lines) and inclusion-loss (blue lines) AS exons, as well as randomly selected constitutive splicing (CS) exons (black lines) for both MXE (left) and SE (right) AS events. It shows that HM changes are significantly different between inclusion-gain and inclusion-loss AS exons (p values, Mann–Whitney–Wilcoxon test). Additional file 1: Figure S5B provides the whole significances of all HMs across AS types and cell lineages. b Pearson correlation test between differential HM signals (ΔHMs) and differential inclusion levels (ΔPSIs), taking H3k36me3 as an example. Additional file 1: Figure S5C provides the correlation test results of other HMs based on two more tests. c A representative k-means cluster shows a subset of SE AS events having a negative correlation between the ΔPSIs and the ΔHMs of H3K36me3. Additional file 1: Figures S5D and S6 provide all the clustering results. d Scatter plot shows that HM-associated AS events display significant correlations between the ΔPSIs and the ΔHMs upon hESC differentiation, taking H3K27ac–associated (positively) MXE events as an example. Also see Additional file 1: Figures S5, S6