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Fig. 5 | Genome Biology

Fig. 5

From: Origin of exon skipping-rich transcriptomes in animals driven by evolution of gene architecture

Fig. 5

Relationship between gene structure and sequence composition and ES. a Heatmap representing the distance between distributions of gene architecture values for IR-positive and IR-negative introns, measured with the D statistic of the one-sided Kolmogorov-Smirnov two-sample test (significant if p < 0.01; otherwise grey). D values are recorded as positive (blue) or negative (red) according to two one-sided Kolmogorov-Smirnov tests with complementary alternative hypotheses. b Heatmap representing genome-level gene architectural traits, for reference: intron density (introns/gene), median exon and intron length (bp), and GC content (percentage). c Volcano plot representing the Spearman’s rank correlation coefficient (rho, x-axis) between IR frequency and transcript expression levels (cRPKM) in IR-positive genes. Significance threshold of p < 0.01 (y-axis, log-scale). d Mean intron–exon GC differential in IR-negative (x-axis) and IR-positive (y-axis) introns. The majority of eukaryotes lie above the diagonal (purple) as they have lower differentials in their IR-positive introns, i.e., IR occurs in GC-homogeneous introns. e Correlation between intron content (total number of introns per genome) and the relative rank of expression of the spliceosomal genes, per species (see “Methods”; Additional file 1: Figure S25)

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