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Fig. 5 | Genome Biology

Fig. 5

From: Comparative transcriptomic analyses and single-cell RNA sequencing of the freshwater planarian Schmidtea mediterranea identify major cell types and pathway conservation

Fig. 5

Smed-egr-5 is required for optic cup regeneration. a Heatmap of predicted transcription factors enriched in different clusters: the set of prediction factors significantly differentially upregulated in at least 4/10 clusters are shown, along with the log2 fold change in expression values and raw counts from corresponding cells. b t-SNE plot of Smed-egr-5 expression demonstrating specificity to Cluster 11. c Bright field images of Smed-egr-5(RNAi) animals exhibiting reduced eye pigmentation. d In situ hybridization of tyr-1 and immunohistochemistry for ARR in Smed-egr-5(RNAi) regenerating and intact animals. Smed-egr-5 knockdown animals regenerated significantly fewer tyr-1+ cells, while intact animals were comparable to controls. Images are 20-μm confocal z-stacks through the left eye. Scale bars = 10 μm. e The majority of Smed-egr-5(RNAi) animals displayed normal photoreceptor neuron regeneration and reinnervation by ARR staining following head amputation (70%), although some abnormalities were also observed. Images are 40–50 μm confocal z-stacks. Scale bars = 50 μm. f Smed-egr-5(RNAi) and control(RNAi) animals displayed comparable eye regeneration following right eye resection. Images are 20-μm confocal z-stacks. Scale bars = 50 μm. g At a lower dose of RNAi (3 feeds), Smed-egr-5(RNAi) animals exhibited some optic cup regeneration at 7 dpa (red arrowheads), which were subsequently lost at later time points. n = 10–20 for all experiments

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