Fig. 3

Variance between collapsed and non-collapsed biological and technical replica samples at embryo stage is low. Dispersion plots generated by the DESeq package in R using the estimateDispersions function. Sequences aligned to each miRNA were counted and variance of samples was estimated. Each dot in the plot represents variance between samples for specific miRNA counts. Y-axis presents a dispersion value, which is the difference between samples squared. For example, a miRNA whose expression in different replicas differs by 10% will have a dispersion value of 0.01. X-axis is the mean of normalized counts. All plots are samples generated from embryo developmental stage: a technical replica samples dispersion estimated with collapsed reads, b biological replica samples dispersion estimated with collapsed reads, c technical replica samples dispersion estimated with non-collapsed reads, and d biological replica samples dispersion estimated with non-collapsed reads