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Fig. 5 | Genome Biology

Fig. 5

From: Homeobox oncogene activation by pan-cancer DNA hypermethylation

Fig. 5

Gene-body canyon hypermethylation by dCas9-SunTag-DNMT3A can directly increase oncogene DLX1 expression. a Schematic graph for dCas9-SunTag-DNMT3A DNA methylation editing system. De-activated Cas9 (dCas9) was fused to SunTag epitopes and single-chain variable fragment (scFv) was fused to GFP and DNMT3A to methylate the gene-body canyon and activate gene expression. b Genome browser tracks of gene-body hypermethylated canyons around homeobox gene DLX1 across 30 normal and 35 tumor samples. c CpG DNA methylation level dramatically increased at the gene-body of DLX1 after adding guide RNA DLX1 (gray bar) with induction of dCas9SunTag and scFvDNMT3A, yet the methylation level in gene promoter was not affected. CpG DNA methylation level was calculated based on two biological replicates. Error bars represent mean ± s.e.m. of biological replicates. d qPCR shows significant increase of gene expression of DLX1 with induction of dCas9SunTag and scFvDNMT3A compared to the same cells without induction. P value was computed by two-sided Student’s t-test

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