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Fig. 2 | Genome Biology

Fig. 2

From: Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells

Fig. 2

Gene expression changes upon aging in pro- and pre-B cells. a, b Volcano plots of total RNA (depleted of rRNA) expression changes in aged pro-B (a) and pre-B cells (b). Names of selected genes showing highly significant changes are indicated. Padj: FDR adjusted p value. c Comparison of fold change in gene expression upon aging between pre-B and pro-B cells, showing a similar trend at both developmental stages; Pearson correlation coefficient is indicated. Genes with a significant (FDR adjusted p value < 0.05) age-related change in expression are indicated by a larger point and are colored based on whether this change is observed in pro-B, pre-B cells or both. Dashed lines indicate a log2 fold change of ± 0.2. d Western blot showing changes in IRS1 protein levels between young and aged pro- and pre-B cells, relative to a loading control (βCOP). Protein equivalent of 1 × 106 cells per lane. Top: A representative western blot is shown. Bottom: Results of quantification of 3 technical repeat westerns from 3 biological repeats (sorts), using Aida software. Data (ratio of relative IRS1 levels:relative βCOP levels) are normalized to young pre-B cells and are means ± SD. Statistical analysis on raw data was with ANOVA combined with Bonferroni’s multiple comparison test. *p = 0.0278; **p = 0.0199

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