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Fig. 1 | Genome Biology

Fig. 1

From: Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells

Fig. 1

Reduction in average pro-B and pre-B cell numbers upon aging. a Representative flow cytometry plots showing the gating strategy used to isolate pro- and pre-B cells from young and aged bone marrow following depletion of non-B cells. Numbers indicate percentage of cells in the gate. bd Average total number of cells (b) and numbers of flow-sorted pro-B (c) and pre-B (d) cells obtained from the bone marrow of both tibias and femurs of young and aged mice. Each point represents the average number of cells per mouse from a single flow sort comprising cohorts of 12–15 mice. Differences were tested for significance using an unpaired t test. e t-SNE analysis based on flow cytometry data shows changes in bone marrow derived B cell precursor populations between young (3 months) and aged mice (19–22 months)

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