Fig. 7

Direction of asymmetric dynamics correlates with direction of transcription. a i Left panel shows a schematic representation of unidirectional expansions that expand in the same direction as transcription and in the opposite direction of transcription. The adjacent plots show, for each mark, the average log₂ ratio across all time points in each time course between the fraction of unidirectional expansions that expand in the same directions as transcription of the nearest gene and the fraction of unidirectional expansions that expand in the opposite direction of transcription of the nearest gene, separately for blocks that are within 1 kb of annotated TSSs and for more distal blocks. Positive values correspond to enrichment of unidirectional expansions in the same direction as transcription. For marks mapped in at least six time courses, a black line is plotted representing the average across all data sets and significant differences are denoted with asterisks based on a two-tailed Mann-Whitney test at a P value threshold of 0.05. ii Left panel shows analogous schematic for unidirectional contractions. Likewise, adjacent plots show, for each mark, the average log₂ ratio between the fraction of unidirectional contractions that contract in the opposite direction of transcription of the nearest TSS and unidirectional contractions that contract in the same direction as transcription of the nearest TSS. b Left panel shows an example of unidirectional expansions between pairs of time points that expand in the same direction as transcription at the Hs6st1 gene of the H3K4me2 mark in the T-cell development dataset [17]. Right panel shows an example of unidirectional contractions in the opposite direction of transcription at the DNMT3B gene. Time points 1, 2, and 3 correspond to in vitro differentiated T-cell precursors, whereas time points 4 and 5 correspond to in vivo purified thymocytes. The predicted ChromTime peaks colored by their boundary dynamics are shown under the signal track for each time point