Evolutionarily conserved partial gene duplication in the Triticeae tribe of grasses confers pathogen resistance

Table 3 Effect of TIGS and transient over-expression (OEX) of HvPUB15, HvARM1, and genes encoding their interacting proteins on QR against B. graminis f.sp. hordei

Bombarded gene	Proposed function	TIGS			Transient OEX
Bombarded gene	Proposed function	Rel. SI (log2)^a	p (t test)^b	n ^c	Rel. SI (log2)^d	p (t test)^b	n ^c
HORVU3Hr1G113910	U-box/ARM E3 protein ligase (HvPUB15)	0.17 ± 0.41	0.6950	5	0.26 ± 0.19	0.2316	7
HORVU3Hr1G081380	ARM-repeat protein (HvARM1)	1.16 ± 0.22	0.0126	4	−0.01 ± 0.21	0.9516	5
HORVU2Hr1G041260	Thylakoid formation 1 (Thf1)	−1.35 ± 0.59	0.0689	6	0.47 ± 12.1	0.0035	6
HORVU2Hr1G003460	ATP-dependent Clp-protease adaptor (ClpS1)	−0.60 ± 0.41	0.2168	5	0.70 ± 0.06	8.43E-5	6
HORVU7Hr1G008760 ^e	Syntaxin HvSNAP34	1.14 ± 0.25	0.0050	5
TaPrx103 ^f	Class III peroxidase TaPrx103				−1.06 ± 0.15	6.61E-6	14

^aRelative to the pIPKTA30 internal empty vector control
^bOne-sample t test (two-tailed) of log2-transformed relative susceptibility index (SI) against the hypothetical value “0”.
^cNumber of independent bombardment experiments
^dRelative to the pIPKTA09 internal empty vector control
^eTIGS of this target gene enhances susceptibility to Bgh and served as positive control [93]
^fOver-expression of this gene enhances resistance in barley and wheat against B. graminis and served as positive control [106]
Statistically significant effects are highlighted in bold

ISSN: 1474-760X