Fig. 6

Genome-wide analysis of expressed and conserved gene duplicates. a Pairs of barley cDNA encoding full-length proteins and partial copies, respectively, which are conserved across Triticeae, were aligned using MAUVE algorithm. The dark green shading in ARM1 or yellow shading in the rest indicates aligning sequences, whereas gray shading shows gaps. Black and red arrowheads indicate start- and stop-codon positions of the corresponding open reading frames, respectively. Non-aligning sequences (in black) at the beginning or end were automatically detached from the alignment blocks. Only the first 500 bp of the CASP-like duplicates giving rise to truncated proteins are shown.  Scale below alignments, sequence length in bp; PD partial duplicate. Aet, Aegilops tauschii; Tu, Triticum urartu; Sc, Secale cereale; Hv, Hordeum vulgare. For annotation and further details see Additional file 2: Table S4. b Transcript regulation in peeled barley leaf epidermis by Bgh (adapted host pathogen) or Bgt (nonhost pathogen). Total RNA was isolated at the time indicated after inoculation and hybridized to the Barley Gene Expression Array of Agilent. For the link of cDNA accession number to Agilent probe IDs, see Additional file 2: Table S4. Transcript data have been submitted to ArrayExpress (Acc. E-MTAB-2916). Hierarchical clustering of gene-median-centered, normalized signal intensities is shown. The color scale ranges from log(2)-1.5 to 1.5. Mean signal intensities from three independent inoculation experiments are shown