Fig. 5

Co-immunoprecipitation (Co-IP) of H. vulgare proteins interacting with HvARM1 and HvPUB15 in vivo. Co-IP of antibody-tagged barley proteins in A. thaliana mesophyll protoplasts. YFP-fused HvARM1 (77.5 kD), and HvPUB15 (83.2 kD) were co-expressed with cMyc-tagged HvThf1 (40 kD) and HvClpS1 (30 kD), respectively, for each interaction. Co-IP was performed using anti-YFP antibodies and total proteins extracted from A. thaliana protoplasts