Fig. 4

Bimolecular functional complementation (BiFC) of YFP by H. vulgare proteins interacting with HvARM1 and HvPUB15 in vivo. BiFC by HvPUB15- and HvARM1-interacting proteins in Nicotiana benthamiana leaves after infiltration of A. tumefaciens strains carrying the protein interaction partners fused C-terminally to split halves of YFP. BF bright field, -SP with deleted N-terminal plastid import signal, VenN N-terminal half of the stabilized YFP version “Venus”, VenC C-terminal half of the YFP “Venus”. Scale bars 20 μm