Fig. 2

Single-cell or single-molecule measurement reveals the stochasticity in splicing kinetics and splice-site choice. a Schematic of stochasticity in splicing kinetics. By labeling the intron with MS2 stem loops (green), the splicing stochasticity can be recorded through the fluorescence fluctuations at the transcription site. b In this histogram, the splicing kinetics of a gene exhibit an exponential distribution, indicating a stochastic process. In this simple stochastic scenario, the most likely splicing time is the shortest measurable time. c Comparing RNA-seq reqd densities from single cells (blue) to a population of cells (gray). Two representative genes, Irgm1 and Clec7a, each with two splicing isoforms (bottom) are shown. Single cell RNA-seq revealed distinct splicing patterns in individual cells. d Distribution of exon inclusion ratios (Percent Spliced in (PSI) scores, x-axis) for alternatively spliced exons in single cells (blue) and population cells (gray). Single-cell RNA-seq reveals a bimodal distribution of splicing isoforms, which is otherwise measured as a normal distribution with different splicing efficiencies from population cells