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Fig. 5 | Genome Biology

Fig. 5

From: A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice

Fig. 5

Evaluate off-target effect of Cas9 and Cpf1 in T1 plants. a, b Analysis of new SNVs and indels in T1 plants. c, d Analysis of SNVs and indels in T1 plants that carry (+) or do not carry () the Cas9 and sgRNA expression cassettes. e Allele frequencies of SNVs and indels identified in all tissue culture-related controls (tissue culture and transformants with Cas9 and Cpf1 backbones), T0, T1, and WT plants. Above: SNVs. Bottom: indels

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