Fig. 4

Analysis and identification of potential off-target sites in T0 lines. a Number of off-target sites identified in replicated T0 plants vs the number of all off-target sites that are predicted by CRISPOR and Cas-OFFinder with allowing up to 3-nt mismatch for all 15 Cas9 or Cpf1 target sites. b Identification of shared SNVs and indels between replicated T0 plants. c Potential off-target sites identified in both Cas9-J T0 samples (above the red dashed line) and only in one T0 sample (below the red dashed line). d Off-target mutations identified by WGS at off-target sites in both Cas9-J T0 samples (above the red dashed line) and only in one T0 sample (below the red dashed line). e Potential off-target sites identified in Cas9-E samples based on shared mutations in two T0 plants. f Sequence analysis of the shared mutations in Cas9-E samples