Detailed analysis of mutations at Cas9- or Cpf1-edited T0 plants. a, b Average numbers of SNVs and indels detected in 26 T0 plants edited by Cas9 or Cpf1. c, d Average numbers of SNVs and indels in edited T0 plants with different numbers of T-DNA insertions. e, f Average numbers of SNVs and indels in Cas9-edited T0 plants expressing one or two sgRNAs (in Cas9-J and Cas9-K). g, h Pearson correlation between on-target editing frequency and the numbers of SNV or indel mutations in Cas9- and Cpf1-edited T0 plants. Error bars in a–f indicate s.e.m.