TY - JOUR AU - Tang, Xu AU - Liu, Guanqing AU - Zhou, Jianping AU - Ren, Qiurong AU - You, Qi AU - Tian, Li AU - Xin, Xuhui AU - Zhong, Zhaohui AU - Liu, Binglin AU - Zheng, Xuelian AU - Zhang, Dengwei AU - Malzahn, Aimee AU - Gong, Zhiyun AU - Qi, Yiping AU - Zhang, Tao AU - Zhang, Yong PY - 2018 DA - 2018/07/04 TI - A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice JO - Genome Biology SP - 84 VL - 19 IS - 1 AB - Targeting specificity has been a barrier to applying genome editing systems in functional genomics, precise medicine and plant breeding. In plants, only limited studies have used whole-genome sequencing (WGS) to test off-target effects of Cas9. The cause of numerous discovered mutations is still controversial. Furthermore, WGS-based off-target analysis of Cpf1 (Cas12a) has not been reported in any higher organism to date. SN - 1474-760X UR - https://doi.org/10.1186/s13059-018-1458-5 DO - 10.1186/s13059-018-1458-5 ID - Tang2018 ER -