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Fig. 8 | Genome Biology

Fig. 8

From: Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells

Fig. 8

Flowchart to guide CRISPR-Cas users in selecting the appropriate system for their experiments. The choice of Cas endonuclease depends on several considerations, such as the type of genome modification desired or the type of repair template to be utilized. For HDR-mediated editing with ssODNs, we recommend asymmetric donors that are complementary to either the target or non-target strand when used in combination with LbCpf1 or SpCas9, respectively

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Genome Biology

ISSN: 1474-760X

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