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Table 1 Mutation frequencies induced by PABE-7 in the T0 rice and wheat plants

From: Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Species Target site sgRNA form Number of mutant lines/plantsa Number of transgenic rice lines or bombarded embryos of wheat A•T to G•C frequency (%)b Genotype of mutations Heterozygous/homozygous
Rice OsACC-T1 sgRNA 9 130 6.9 T4 > C4 (2); T4T7 > C4C7 (7) 9/0
esgRNA 33 160 20.6 T4 > C4 (10); T7 > C7 (2); T4T7 > C4C7 (21) 32/1
OsALS-T1 sgRNA 16 184 8.7 A5 > G5 (16) 16/0
esgRNA 42 196 21.4 A4 > G4 (1); A5 > G5 (41) 42/0
OsCDC48-T3 sgRNA 19 210 9.0 A5 > G5 (19) 19/0
esgRNA 60 180 33.3 A5 > G5 (60) 60/0
OsDEP1-T1 sgRNA 101 217 46.5 A4 > G4 (2); A6 > G6 (90); A4A6 > G4G6 (9) 88/13
esgRNA 83 211 39.3 A4 > G4 (4); A6 > G6 (73); A4A6 > G4G6 (6) 77/6
OsDEP1-T2 sgRNA 5 154 3.2 A6 > G6 (5) 5/0
esgRNA 34 215 15.8 A5 > G5 (1); A6 > G6 (32);
A3A6 > G3G6 (1)
33/1
OsNRT1.1B-T1 sgRNA 116 303 38.3 A6 > G6 (8); A8 > G8 (30); A4A8 > G4G8 (3); A6A8 > G6G8 (75) 111/5
esgRNA 149 252 59.1 A6 > G6 (6); A8 > G8 (46); A4A8 > G4G8 (2); A6A8 > G6G8 (95) 136/13
Wheat TaDEP1 esgRNA 5 460 1.1 A8 > G8 (4, AaBBDD;
1, AABbDD)
5/0
TaGW2 esgRNA 2 480 0.4 A5 > G5 (2, AABbDD) 2/0
  1. aThe number of mutant lines for rice and the number of mutant plants for wheat. b Based on the number of T0 lines (rice) or plants (wheat) carrying the observed mutations over the total number of T0 transgenic rice lines analyzed or bombarded immature embryos of wheat