Fig. 4

Splice site control sequences generally reflect expected splicing behavior. Boxplots of mean Ψ for each type of control and test sequences are shown. Mutated splice site controls contained mutated splice sites such that the 3′ splice sites were changed from AG to TC and the 5′ splice sites were changed from GT to CA. For the consensus splice site controls, the variants contained a 20-nucleotide pyrimidine tract, an AG at the 3′ splice site, and a consensus 5′ splice site of GTAAGT