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Fig. 2 | Genome Biology

Fig. 2

From: Vex-seq: high-throughput identification of the impact of genetic variation on pre-mRNA splicing efficiency

Fig. 2

Quality control for plasmid integrity. a Quality control pipeline for plasmid integrity. Amplicon sequencing of the 1o and 2o plasmid configurations are done through PCR to attach Illumina flow cell binding sequences (FC) and indexes (I1 and I2). Poor quality barcodes are then filtered out by identification of reads not containing variants and excluding barcodes with less than 85% of reads containing the correct variant. b A histogram of the barcodes with the percentage of reads with correct variant identified. c Box plots of 1˚ library read depth for barcodes included and excluded from further analysis

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