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Fig. 6 | Genome Biology

Fig. 6

From: Ythdf2-mediated m6A mRNA clearance modulates neural development in mice

Fig. 6

Ythdf2 is required for regulating mRNA decay of m6A-modified neuron-related gene targets. a m6A enrichment of target sites in gene candidates, verified by m6A IP combined with RT-qPCR. Non-m6A-modified gene Actin was used as negative control. b Gene expression of gene candidates, verified by RT-qPCR with input RNA. Non-changed gene Actin was used as negative control. c Ythdf2 binding levels of gene candidates, verified by Ythdf2 RIP combined with RT-qPCR. Non-m6A-modified gene Actin was used as negative control. d Representative mRNA profile of Nrp2 at 0-, 2-, and 4-h time points after actinomycin D (5 μg/ml) treatment (h.p.t.) in wild type and Ythdf2−/−. Error bars represent mean ± standard deviation, n = 2 biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test

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