Fig. 3

The number of basal progenitors and mitotic capability of apical progenitors depends on Ythdf2. a Immunostaining of E12.5 and E14.5 sagittal sections with Tbr2 (green) and Sox2 (red) antibodies in wild type, Ythdf2^+/−, and Ythdf2^−/− embryos. VZ ventricular zone, SVZ subventricular zone, IZ intermediate zone, CP cortical plate. Nuclei were counterstained with DAPI. b Percentage of Tbr2⁺ cells over Tbr2⁺/Sox2⁺ at E12.5 and E14.5. Error bars represent mean ± standard deviation, n = 3 biological and 3 technical replicates. Scale bars, 20 μm. c Immunostaining of E12.5 and E14.5 sagittal sections with Phh3 (green) and Sox2 (red) antibodies in wild type, Ythdf2^+/−, and Ythdf2^−/−embryos. Nuclei were counterstained with DAPI. d Number of Phh3⁺ cells per 400 μm of the cortical wall at E12.5/E14.5 from c. Error bars represent mean ± standard deviation, n = 3 biological and 3 technical replicates. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test. Scale bars, 20 μm