Fig. 5

Defective cerebellar development in Alkbh5 knockout mice after being exposed to hypobaric hypoxia for 48 h. a Brain weight of individual wild-type (WT) and Alkbh5 knockout (KO) mice. Numbers of biological replicates are included in parentheses. Black lines in the graph indicate the mean. ***P < 0.001. b Representative HE staining images of wild-type (WT) and Alkbh5 knockout (KO) mouse cerebellum (P7). n = 7 for WT and KO, respectively. Scale bar, 100 μm. c–f Representative images from immunostaining analysis with antibodies against Ki67 (c), phospho-H3 (PH3) (d), BrdU (e), and NeuN (f) in the cerebellum of WT and KO mice. Quantification of immuno-reactive cells is shown in the right panels. Numbers of biological replicates are included in parentheses. Black lines in the graph indicate the mean. *P < 0.05, **P < 0.01, ns not significant. Sections in c and e were stained with DAPI and sections in d and f were counterstained with eosin to visualize nuclei. Scale bar, 100 μm. g, h Representative images from immunohistochemical analysis with antibodies against Calbindin-D28K (g) and GFAP (h) to detect Purkinje cells (g) and astrocytes (h). n = 7 for WT and KO, respectively. Scale bar, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant