Fig. 3

Dynamic expression of m⁶A-related genes and RNA methylation levels during mouse cerebellar development. a Western blot analysis showing the expression levels of m⁶A methyltransferases and demethylases in the mouse cerebellum from P7 to P60. n = 12 biological replicates. GAPDH is used as an internal control. b, c Immunostaining of mouse cerebellum at P7 and P60 using antibodies against the five m⁶A-related proteins. n = 4 biological replicates. Enlarged images in the dashed boxes are shown in c. Scale bar in b represents 50 μm, and scale bar in c represents 10 μm. EGL external granule cell layer, IGL internal granule cell layer, PCL Purkinje cell layer, ML molecular layer. The dashed circles indicate the representative Purkinje cells. d Dynamic poly(A) RNA m⁶A methylation levels during mouse cerebellar development. Mouse cerebella at P7, P14, P21, and P60 were included in this assay. Experiments were repeated three times using 15 mice in total. Representative data are shown here. ***P < 0.001