Fig. 1

Open chromatin enrichment and network Hi-C (OCEAN-C) identifies hubs of open chromatin interactions (HOCIs) without the need for antibodies. a The OCEAN-C method. b Venn diagram of peaks determined by FAIRE-seq, OCEAN-C, and Hi-C methods in U266 cells using the ZINBA algorithm. c Boxplots showing the distributions of OCEAN-C and Hi-C reads in OCEAN-C peaks of U266 cells. d An example of chromatin interactions involving HOCIs. The browser view of a 1.5-Mb region shows a randomly chosen HOCI with associated OCEAN-C and Hi-C interactions. Each loop indicates a unique valid read pair. e Percentage of peaks or random regions of OCEAN-C, Hi-C, and FAIRE-seq that overlap with various histone modification markers (U266). f Venn diagram of HOCIs called from three cell lines (U266, RPMI-8226, and GM12878)