Fig. 6

YY1 and HDAC1 role in inter-individual behavioral variability and acH4 changes. a The most represented motifs found in the acH4 peaks located near TSS. The predicted transcription factors that can bind to these sites are also indicated if found, with the percentage of the total peaks that present at least one of these motifs. b Probability density map for the behavior of 24 yy1 +/+ (left) and yy1 +/− (right) fish. c AcH4 (dark blue), acetyl-H4K12 (light blue) and additional control IgG (red) levels found in yy1 +/+ and yy1 +/− larvae, as detected by fold change compared to an unbound fraction in eight selected regions that are shown in the legend on the right. d The same as b but for yy1 +/− and yy1 +/− treated with 2 mM NaBu for 24 h. e YY1 binding (blue dots) and additional IgG presence (red dots) to the eight selected regions quantified by fold change compared to the unbound fraction in eight selected regions in AB (control), NaBu-treated, hdac1 +/−, and yy1 +/− larvae. f Same as e but for HDAC1 binding. g ReChIP fold change in control AB larvae. The order of the two consecutive ChIPs is noted in the names of the conditions. h YY1 binding in eight selected regions to clusters of larvae with different distances r to the average behavior of the population. i Same as h but for HDAC1 binding. j YY1 acetylation in control AB, NaBu-treated, hdac1 +/−, and yy1 +/− larvae. YY1-immunoprecipitated extracts were subjected to western blot analysis using an acetylated-lysine antibody (top left) or YY1 antibody (bottom left). Quantification of the acetyl-YY1/YY1 ratio is shown on the right