Fig. 4

Relation between histone acetylation levels and behavior. a Average histone H4 acetylation levels of fish depending on their behavior using 90 fish as the initial population and clustering them into groups (left). The polar coordinate system as a transformation of the previous Cartesian system, and the two parameters used to analyze the dependence between histone H4 acetylation and behavior (right). b Relation between the values of histone H4 acetylation and the two parameters of the polar coordinate system centered on the average behavior of the population: the distance to the average (left) and the angle with the horizontal axis (right). Blue dots are control larvae while red dots indicate Nabu-treated animals. R coefficients for control samples are shown. c Same as b, but for histone H3 acetylation. d Relation between the values of histone H4K5 (top left), H4K8 (top right), H4K12 (bottom left), and H4K16 (bottom right) acetylation and the distance to the average behavior of the population. R coefficients are shown. e Relation between the values of histone H4 (left) and H3 (right) acetylation and clusters of larvae with similar intra-individual behavioral variability