Fig. 1

Overview of scRNA-seq analysis on hPSC early differentiation. a Process flow diagram of scRNA-seq analysis on hPSC early differentiation. Single-cell samples of Naïve-like H9, Primed H9, and EBs were prepared by Fluidigm C1 system with HT IFCs for sequencing. Data analysis was performed using Seurat and Monocle. b Violin plots show the distribution of transcripts and genes detected per cell. c t-SNE plot of single-cell samples profiled. Naïve-like H9 cluster (blue circle), EB clusters (black circle), Primed H9 cluster (red circle)