Fig. 4

Dex treatment does not induce detectable changes in histone methylation. a Enrichment of methylated H3K IP over unmodified H3 IP for annotated features. Only features with at least 1.2-fold enrichment or depletion in one or more sample are shown. Error bars represent range for the three replicates. No significant differences were observed (Student’s t-test, Benjamini-Hochberg adjusted p-value, 10% FDR). b Enrichment of methylated H3K IP over unmodified H3 IP centred over transcription start sites (TSS) and transcription termination site (TTS) ± 3000 bp. Each replicate is shown as a separate line. c Hierarchical clustering of samples and peaks by average enrichment of methylated H3K IP over unmodified H3 IP. Samples clearly cluster by histone mark but do not cluster by Veh vs Dex for any histone mark. Vertical colour bar indicates six clusters following k-means clustering. Gold and purple clusters show higher H3K9me3 enrichment. Bivalent enrichment observed for H3K4me3 and H3K9me3 (turquoise) and H3K4me3 and H3K27me3 (green). Blue cluster represents inactive enhancers marked by H3K4me1 and H3K9me3