Fig. 3

Small RNA expression in the F1 sperm is unaffected by Dex treatment. a The proportion of reads aligning to annotated small RNA species. Replicate samples are shown separately. b Length profile of sRNA-Seq reads following trimming to remove adapter read-through sequences. Reads exceeding 38 bp are not shown. Replicates are shown as separate lines. c Hierarchical clustering of Veh and Dex samples based on miRNA expression. Spearman’s correlation Rho shown below in heatmap. d Expression of candidate miRNAs in total RNA from sperm (n = 8/8). No significant differences were observed (Student’s t-test)