Fig. 7

Responses of neural and glioma stem cells to differentiation cues. a Representative IF images for NSC27 cultured under neuronal- (NDM) and astrocytic-induction conditions (ADM). Bright field images (top) showing cell morphology. Scale bar is indicated. The indicated proteins are stained in green. DAPI indicates nuclear staining in blue. b Expression of DNMTs and TETs before and after differentiation induction in NSC27, expressed as mean ± SEM. X-axis shows the gene. Y-axis shows the normalized (to DYNLL) mRNA expression. * P < 0.05. c Correlation of differentiation-induced expression changes among DNA modification machinery genes and stem/lineage markers. Blue: positive correlation; orange: negative correlation. Dot size represents the magnitude of correlation. Genes within the same box are most correlated with each other. d IF images for stem and lineage markers in GSC84 cultured under serum-free stem conditions (left) and 10% FBS differentiation-inducing conditions (right). NESTIN is stained in red; SOX-2, GFAP, and TUBB3 are stained in green. Scale bar: 400 μm. e IF images for TET1, TET2, TET3 (top), and GFAP (bottom) in 10% FBS conditions in GSC84. The white dashed box indicates GFAP expression is absent in cells with nuclear TET3 expression. Scale bar: 200 μm. f Stacked bar graph showing the percent cells displaying only nuclear TET3 or cytoplasmic TET3 in the absence/presence of GFAP. Representative IF images of GFAP and TET3 are shown at the right. White dashed box highlights a region where GFAP expression is absent when TET3 staining is nuclear