Fig. 6

BIFC-dCas9/gRNA strategies showed high signal-to-noise ratio and no nonspecific foci. a Labeling of telomeres by different BIFC approaches. Left panel: the telomeres were labeled by Venus while the mCherry-TRF1 was used as control. Right panel: Quantification of telomere labeling specificity by co-localization with mCherry-TRF1 signals. b Comparison of different BIFC-dCas9/gRNA systems for labeling the endogenous human MUC4 genomic locus. c Dot plots of signal-to-noise ratio in different BIFC-dCas9/gRNA genome-labeling systems in comparison with SunTag system. The same amounts of dCas9, gRNA, scFv-Venus, or scFv-VenusC/N expression constructs were transfected for different methods. The data are displayed as mean ± SD. Unpaired t test was used. ***p < 0.001, ****p< 0.0001