Fig. 5
From: Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
Schematic views of the different BIFC-dCas9/gRNA genome-labeling systems. a In scFv-BIFC, scFv-VenusN and scFv-VenusC are recruited to the SunTag of dCas9 where a functional Venus could be assembled. b In dCas9-MCP-BIFC, the VenusN is fused with MCP, which could be recruited to the dCas9/gRNA complex where it could interact with the VenusC fragment fused with dCas9 to form a functional Venus molecule. c In SunTag-dCas9-MCP-BIFC, the VenusC fragment is fused with scFv to be recruited to dCas9