Fig. 8

SORBS2 depletion-induced secretome alterations are associated with monocyte to MDSC and M2-like macrophage polarization in human. a A schematic model of the monocyte polarization protocol. CD14+ cells were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) via magnetic CD14+ microbeads and incubated with conditioned medium (CM) from control A2780s ovarian cancer cells, SORBS2-depleted A2780s ovarian cancer cells, WFDC1-overexpressing SORBS2-depleted A2780s ovarian cancer cells, and IL-17D-overexpressing SORBS2-depleted A2780s ovarian cancer cells. After 48 h, treated myeloid cells were stained with fluorochrome-labeled antibodies against HLA-DR, CD14, and CD206 and analyzed by flow cytometry. Data are presented as fold change in HLA-DRlo/neg CD14+ cells (b), HLA-DRlo/neg, CD14+, CD206+ cells (c), or HLA-DR+, CD14+ CD206+ macrophages (d) upon conditioned media incubation. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001