Method
|
Strategy
|
BS conversion
|
PCR cycles
|
Polymerase
|
Libraries/Studies/Labs
|
---|
Alkaline
|
Pre-bisulfite
|
Alkaline
|
15–18
|
Pfu Turbo Cx
|
18/2/1
|
Heat
|
Pre-bisulfite
|
Heat
|
10–16
|
Pfu Turbo Cx
|
49/4/3a
|
KAPA
|
Pre-bisulfite
|
Heat and alkaline
|
6–15
|
KAPA Uracil+
|
47/4/2
|
Am-BS
|
Pre-bisulfite
|
Am-BS
|
9
|
JumpStart
|
14/2/1
|
PBAT
|
Post-bisulfite
|
Heat
|
Noneb
|
–
|
51/3/2
|
ampPBAT
|
Post-bisulfite
|
Heat and alkalinec
|
5–12b
|
KAPA HiFi
|
23/4/2
|
EpiGnome
|
Post-bisulfite
|
Heat
|
9–10b
|
FailSafe
|
14/2/2
|
- aOne of the ‘Heat’ studies is generated with Hot Start Taq and is only used as part of Set 2 for Fig. 5 and Additional file 2: Figure S6 and S7
- bInclude one, three or five steps of strand synthesis and pre-PCR enrichment (see Additional file 1)
- cThe alkaline procedure has modifications from the manufacturer’s protocol (see Additional file 1)