Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: Genomic positional conservation identifies topological anchor point RNAs linked to developmental loci

Fig. 5

FOXA2-DS-S regulates FOXA2 expression. a Screenshot from the Dalliance genome browser [50] showing the FOXA2 locus with tracks displaying coverage data for ChIP-Seq experiments for Pol2, FOXA1, FOXA2, HNF4A, HNF6 and CEBPA. The ChIP-Seq tracks were produced by the ENCODE project on HepG2 cells. b Real time PCR data showing the expression of FOXA2 and FOXA2-DS-S in Huh7 cells upon knock-down. Si1 and si2 FOXA2-DS-S indicate two different, non-overlapping siRNAs designed against FOXA2-DS-S. The data are expressed relative to the expression of the control transfected with scrambled siRNAs; the error bars indicate the standard error of the mean across three replicate experiments. c Venn diagram showing the number of significantly differentially expressed genes (adjusted p value < 0.05 and log2 fold change > or < 1.25) in the microarray experiment on Huh7 knock-down of FOXA2 or FOXA-DS-S. d Heatmap showing microarray data upon knock-down of FOXA2 or FOXA-DS-S in Huh7 cells. The colour scale indicates normalised intensities (z-score). The heatmap contains all genes that were significantly altered (adjusted p < 0.05) upon knoc- down of either FOXA2 or FOXA-DS-S. The scatter plots in the lower part of the panel show GO enrichment data for genes that were significantly down-regulated (left) or up-regulated (right) in either siFOXA2 or siFOXA-DS-S

Back to article page