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Fig. 3 | Genome Biology

Fig. 3

From: ROP: dumpster diving in RNA-sequencing to find the source of 1 trillion reads across diverse adult human tissues

Fig. 3

Combinatorial diversity of IGK locus differentiates disease status. a Heatmap depicting the percentage of RNA-seq samples supporting of particular VJ combination for whole blood (n = 19), nasal epithelium of healthy controls (n = 10), and asthmatic individuals (n = 9). Each row corresponds to a V gene and each column corresponds to a J gene. b Alpha diversity of nasal samples is measured using the Shannon entropy and incorporates total number of VJ combinations and their relative proportions. Nasal epithelium of asthmatic individuals exhibits decreased combinatorial diversity of IGK locus compared to healthy controls (p value = 1 × 10− 3). c Compositional similarities between the nasal samples in terms of gain or loss of VJ combinations of IGK locus are measured across paired samples from the same group (Asthma, Controls) and paired samples from different groups (Asthma vs Controls) using Sørensen–Dice index. Lower level of similarity is observed between nasal samples of asthmatic individuals compared to unaffected controls (p value < 7.3 × 10− 13). Nasal samples of unaffected controls are more similar to each other than to the asthmatic individuals (p value < 2.5 × 10− 9)

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