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Fig. 2 | Genome Biology

Fig. 2

From: i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

Fig. 2

Creating gene-inactivated animal models using the GONAD method. a Schematic of the targeting strategy to inactivate Foxe3 gene and the primer set used for genotyping. b Direct sequencing results of polymerase chain reaction (PCR) products amplified from the founder (G0) mice with the primer set shown in a. The red arrows below the electropherogram show the region with indel mutations. c Mutated Foxe3 alleles in the G0 mice. The changes in the nucleotide sequence are shown in red, and the type of changes (insertions +Xnt, or deletions Δ) is indicated on the right side of the sequences. d and e Cataract phenotypes in the G1 mice. f Efficiencies of Foxe3 gene editing. CRISPR components used were either Cas9 mRNA/sgRNA or Cas9 protein/CRISPR RNA (crRNA)/trans-activating crRNA (tracrRNA) (see Additional file 1: Table S1 for details)

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