Fig. 5

H3Q85C cleavage data prove linker “quantization.” a A two-dimensional occupancy heat map and length histogram show the coverage of DNA fragments of different sizes relative to +1 nucleosomes. There are three major groups of fragment sizes. Small fragments (~ 50 bp long) originate from two cuts inside the same nucleosome; their centers indicate the position of a nucleosome dyad. Medium fragments (~ 70–130 bp long) and long fragments (~ 135–175 bp long) originate from two cuts inside neighboring nucleosomes, produced at consecutive or alternate cleavage sites, respectively. b Scheme of the cleavage sites and the possible resulting fragments, together with the histogram of DNA fragment lengths obtained in the experiment