Fig. 1

scRNA-seq identifies five response clusters among EC mDCs. a Left: Schematic representation of experimental system. After incubation with virus or a media control for 48 h, mDCs were isolated from PBMCs by FACS and profiled by scRNA-seq. Right: Violin plots of single-cell expression levels for ten select genes for each EC donor (p1, p2, p3). Vertical lines represent individual cellular values; the upper (gray) half of the violin shows the distribution of values for the media control and the bottom (red) shows the same for virus-exposed cells. b t-distributed stochastic neighbor embedding (tSNE) of all FACS sorted mDCs across three EC subjects passing quality filters (see “Methods”; p1: circles, p2: triangles, p3: squares). Virus exposed cells are outlined in red; media exposed cells have no outline. Cells separate into five distinct clusters (c1–5; see “Methods”). c Stacked bar plot depicting the percentage of total mDCs in each cluster for each patient under media and viral exposure conditions