Fig. 2

Induction of canonical Wnt signalling in a subpopulation of cells. a Estimated proportions of state A and state D cells in WT and beta-catenin activated epidermis samples from Collins et al. (GSE32966) [17]. b Immunofluorescence showing cytoplasmic and nuclear beta-catenin in WT and K14ΔNβ-cateninER keratinocytes after induction of canonical Wnt signalling using 4OHT. Scale bar, 20 μm. c Quantification of mean nuclear beta-catenin fluorescence intensity (n = 3 independent cultures). d Activation of canonical Wnt target genes in K14ΔNβ-cateninER cells upon induction with 4OHT quantified by quantitative reverse transcription polymerase chain reaction (n = 4). *P < 0.05, n.s. not significant. All data shown as mean ± SD