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Fig. 2 | Genome Biology

Fig. 2

From: RNA virus interference via CRISPR/Cas13a system in plants

Fig. 2

The pCas13a–crRNA complex interferes with TuMV-GFP in planta. a pCas13a mediates interference with the GFP-expressing TuMV virus in plants. N. benthamiana plants expressing pCas13a were infiltrated with TRV expressing crRNAs and TuMV-GFP. At 7 dpi, plants were imaged under UV light for GFP. The GFP signal of the plants having target crRNAs were compared to plants having no crRNA or a ns-crRNA. b GFP protein detection was used to validate the TuMV-GFP interference. Protein blots from (a) were developed with anti-GFP antibody. The arrow indicates the size of the GFP band. c Northern blot confirms that Hc-crRNA and GFP2-crRNA give better interference with TuMV. RNA blots from (a) were probed with a DIG-labeled TuMV complementary (250-nt) RNA fragment and detected with anti-DIG antibody. The arrow indicates the accumulation of the TuMV RNA genome. In (b) and (c) the lower panels were used as loading controls

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