Fig. 3

Stable transcription is not indicative of enhancer activity. a As shown by the curved arrows, the putative target of each chromatin state locus and bidirectionally transcribed-defined enhancer is defined as the nearest annotated gene (shown in the green boxes). The activity of each locus as measured by either the level of transcription initiation (the bidirectional arrows above each regulatory region) or the strength of the DHS signal (the peaks below each regulatory region) is then correlated with transcription initiation at the putative target gene promoter. b The percentage of chromatin state loci and bidirectionally transcribed-defined enhancers whose measure of stable transcription initiation is significantly correlated with transcription initiation from the nearest annotated gene promoter. The error bars represent the 95% confidence interval from 1000 samplings of the data with replacement, while the numbers below each bar denote the number of loci tested for a significant correlation. c As for b, but the correlations being considered are between the level of DHS signal of chromatin state loci and bidirectionally transcribed-defined enhancers and transcription initiation from the nearest annotated gene promoter