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Table 3 Assignment rules for differentially expressed contigs

From: DE-kupl: exhaustive capture of biological variation in RNA-seq data through k-mer decomposition

 

Conditions

Event class

DU P value

Number of junctions

Maps gene

Maps antisense gene

Clipped 3’

Is mapped

SNV

Exonic

Intronic

Number of hits

Contig length

Other rules

Contigs

Loci

Splicing

 

>0

T

  

T

F

  

1

  

1879

1280

Splicing DU

<0.01

>0

T

  

T

F

  

1

  

391

345

PolyA

    

≥5

T

   

1

 

1

105

95

PolyA DU

<0.01

 

T

 

≥5

T

   

1

 

1

9

8

lincRNA

  

F

F

 

T

   

1

>200

 

1061

329

asRNA

  

F

T

 

T

   

1

>200

 

479

180

SNV DU

<0.01

 

T

F

 

T

T

T

 

1

 

2

929

680

Intron

  

T

F

0

T

  

T

1

 

3

49897

6689

Intron DU

<0.01

 

T

F

0

T

  

T

1

 

3

10688

3128

Repeats

     

T

   

≥5

>50

4

1136

612

Unmapped

     

F

    

>50

 

112

 
  1. Each class of event is defined by a set of rules applied to annotated contigs. Other rules refers to the following: (1) contig ends with AAAAA, (2) mean counts >20 in at least one condition and mapped region <10 kb, (3) mapped region <10 kb, and (4) the mapped gene is not differentially expressed. Contigs indicates the number of contigs of each class found in the epithelial–mesenchymal transition experiment. Loci is the number of loci implicated by these contigs (see “Methods”)
  2. asRNA antisense RNA, DU differential usage, F false, lincRNA long intergenic non-coding RNA, PolyA polyadenylated, SNV single-nucleotide variation, T true