Skip to main content
Fig. 9 | Genome Biology

Fig. 9

From: The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

Fig. 9

miR-497-5p expression in HNSCC patients and its regulation by circPVT1. a miRNA expression analysis as in [40] was used to evaluate miRNA-497-5p expression in tumoral (T) and non-tumoral (N) samples. b miRNA-497-5p level was detected by RT-qPCR in siRNAcircPVT1 and siRNAct transfected cells. c miRNA-497-5p level was detected by RT-qPCR in pcDNA3-circPVT1-a, pcDNA3-circPVT1-b, and pcDNA3 transfected cells. d p53 mRNA level was detected by RT-qPCR in pcDNA3-circPVT1-a, pcDNA3-circPVT1-b, and pcDNA3 transfected cells. e PVT1 level was detected by RT-qPCR in pcDNA3-circPVT1-a, pcDNA3-circPVT1-b, and pcDNA3 transfected cells. f circPVT1 binding site on miR-497-5p. g circPVT1 level was detected by RT-qPCR after the transfection of the indicated vectors and mimics. h miR-497-5p level was detected by RT-qPCR after the transfection of the indicated vectors and mimics. Data are shown as mean of three replicates ± standard deviation (Student’s test; *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant)

Back to article page