Fig. 6From: Single-cell profiling of human gliomas reveals macrophage ontogeny as a basis for regional differences in macrophage activation in the tumor microenvironmentTAMs simultaneously co-express canonical M1 and M2 markers in individual cells. a–c Distributions of canonical M1 and M2 marker genes, in cells expressing IL10, compared across scRNA-seq platforms. d Flow cytometric analysis of tumor-infiltrating CD206 + CD86+ TAMs gated on live CD11b + myeloid cells. e Representative flow cytometric analysis of tumor-infiltrating CD204+ TLR2+ cells gated on CD11b+, CD49D+ live macrophages (left) and CD11b+, P2RY12+ live microglia (right). f Quantification of flow cytometric analysis. Cells positive for indicated markers are given by circles (n = 3 patients). The fraction of cells positive for each individual marker is given by the histogram on the left, the fraction of cells positive for each marker combination is given on top of each panelBack to article page